Projects

This research project aims to investigate how exosomes produced by Salmonella-infected antigen-presenting cells (APCs) drive adaptive immunity, focusing on their effects on Ag-specific immune responses. The initial experiment involves generating recombinant proteins of select Salmonella antigens (Ags) like OmpA, SopB, and CirA, which will serve as key components for further experimentation. These proteins will be prepared using molecular biology techniques. The subsequent experiment aims to assess the effects of exosomes derived from Salmonella-infected macrophages (MΦs) on Ag-specific humoral immune responses in mice. Immunization with exosomes will be followed by analyzing antibody responses against specific antigens using ELISA. The study will also measure antigen-specific IgA antibodies in mouse feces and secretory IgA production in Peyer’s patches. This research, contributing valuable insights into Salmonella-induced immunity mechanisms, could potentially influence the development of novel vaccination strategies against Salmonella infection and related diseases.